Research Article
Physicochemical and Structural Validation of Myxovirus Resistance 1 (Mx1) Protein of Three Strains of the Nigerian Indigenous (Gallus Gallus domesticus) and Exotic Chickens
Yeigba Bolouinbele Japhet*,
Toipre Omiete Samuel,
Kai Woyingiemi Bolouzimo
Issue:
Volume 9, Issue 2, December 2024
Pages:
25-41
Received:
4 September 2024
Accepted:
27 September 2024
Published:
18 October 2024
Abstract: This research was conducted on three strains of Nigerian Indigenous (gallus gallus domesticus) and Noiler (exotic) chickens. It examined the physicochemical and structural validation of Myxovirus resistance 1 (Mx1) protein on the three strains of Nigerian indigenous (naked neck, frizzle feather, normal feather) and noiler (exotic) chickens and also the prediction of the Physicochemical Analysis of protein. Nucleotide sequence were retrieved from National Center for Bio-Technology Information (NCBI) database and subjected to multiple sequence alignment, prediction of the physicochemical analysis of protein was done in the ProtParam web server. Modeling of 3D structural validation, Swiss modeling and Statistical analysis were all carried out. Software was used to align the sequences to find any Single Nucleotide Polymorphism (SNPS). The result of physicochemical analysis showed that the properties of the Mx1 protein fell within accepted threshold and predicted that the proteins were generally related. To validate the structure obtained from modeling, the obtained PDB files were ran on the Pro-check validation server and obtained Errat and Ramachandran plots. The study elucidates the unique features and potential functional implications of Mx1 protein across different strains of Nigerian indigenous and exotic chickens. This knowledge can inform the development of strategies to improve disease resistance in local chicken populations through selective breeding or genetic manipulation. Comparing the Mx1 protein among different strains of Nigerian indigenous chickens can reveal evolutionary adaptations and provide valuable information for understanding the molecular basis of immune defense mechanisms in poultry. Understanding the physicochemical properties and structural dynamics of Mx1 protein contributes to the broader understanding of innate immune responses in indigenous chicken breeds, offering insights into their disease resistance and adaptation mechanisms.
Abstract: This research was conducted on three strains of Nigerian Indigenous (gallus gallus domesticus) and Noiler (exotic) chickens. It examined the physicochemical and structural validation of Myxovirus resistance 1 (Mx1) protein on the three strains of Nigerian indigenous (naked neck, frizzle feather, normal feather) and noiler (exotic) chickens and also...
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Review Article
Exploring the Role of Short Tandem Repeats (STR) in Forensic Biotechnology: Challenges and Innovations
Betelhem Abebe*
,
Temesgen Mitiku,
Nega Birhane
Issue:
Volume 9, Issue 2, December 2024
Pages:
42-49
Received:
26 October 2024
Accepted:
5 December 2024
Published:
25 December 2024
DOI:
10.11648/j.ijbbmb.20240902.12
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Abstract: Short tandem repeat (STR) typing remains a crucial tool in forensic DNA profiling worldwide. This technology not only helps in convicting criminals and proving wrongful convictions but also establishes essential links to actual perpetrators of crimes and can act as a deterrent to potential offenders. DNA profiling has also allowed forensic scientists to re-examine closed cases due to insufficient evidence. To conduct this review, an extensive electronic literature search was performed using databases such as PubMed, Science Direct, Google Scholar, and Google Search. All relevant works, including reviews, retrospective studies, observational studies, and original articles, were thoroughly reviewed. This paper explores the challenges and perspectives associated with using STRs in forensic investigations. STRs have high polymorphism, Mendelian inheritance, and the ability to analyze multiple loci in one reaction, making them essential tools in forensic science. Additionally, their suitability for analyzing degraded or traces DNA samples enhances their usefulness in criminal investigations and paternity testing. This abstract summarizes the current landscape of STR applications in forensic biotechnology, highlighting the challenges researchers face and the promising features that drive innovation in this field.
Abstract: Short tandem repeat (STR) typing remains a crucial tool in forensic DNA profiling worldwide. This technology not only helps in convicting criminals and proving wrongful convictions but also establishes essential links to actual perpetrators of crimes and can act as a deterrent to potential offenders. DNA profiling has also allowed forensic scientis...
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